PD Dr. Bert Engelen ...
... wurde 1963 in Duisburg geboren
und ist seit 2001 Wissenschaftlicher Assistent in der Arbeitsgruppe Paläomikrobiologie
des Instituts für Chemie und Biologie des Meeres (ICBM) an der Universität Oldenburg.
Er studierte in Braunschweig Biologie mit den Schwerpunkten Mikrobiologie, Biochemie und Siedlungswasserwirtschaft.
Im Jahr 1998 promovierte er an der Biologischen Bundesanstalt für Land- und Forstwirtschaft und arbeitete anschließend als Postdoc bei der Gesellschaft für Biotechnologische Forschung in einem dreijährigen Forschungsprojekt.
Neben seiner Lehrtätigkeit an der Universität liegen seine Forschungsschwerpunkte in der Analyse von mikrobiellen Gemeinschaften mariner Sedimente, aber auch anderer Standorte wie z.B. des Bodens, der Wassersäule sowie in Kohle- Gas- und Erdöllagerstätten.
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... was born in 1963 in Duisburg, Germany. Since 2001, he is working as a research associate in the "Paleomicrobiology group"
at the "Institute for Chemistry and Biology of the Marine Environment" (ICBM) at the University of Oldenburg.
He has studied biology in Braunschweig, Germany with microbiology, biochemistry and sanitary environmental engineering as main subjects. He made his Phd in 1998 at the "Federal Biological Research Centre for Agriculture and Forestry" and continued working for three years as a postdoc at the "Federal Research Centre for Biotechnology" (GBF).
Besides his teaching duties at the university, his main research interest is the analysis of microbial communities in marine sediments, but also in other habitats such as soil, the water column, or coal-, gas-, and oil deposits.
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Scientific education and positions |
2016
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Habilitation: "Prokaryotes and viruses in the marine subsurface:
From coastal sediments to the deep-subseafloor biosphere", Venia legendi for Microbiology, University of Oldenburg, Germany
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2001 - present
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Research Associate, Paleomicrobiology group, Inst. for Chemistry and Biology of the Marine Environment (ICBM), University of Oldenburg, Germany
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1998 - 2001
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Postdoctoral fellow, EU-Project: Marine Bacterial Genes and Isolates as Sources for Novel Biotechnological Products (MARGENES), Fed. Res. Centre for Biotechnology (GBF), Braunschweig, Germany
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1993 - 1997
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PhD-thesis: Development and application of methods to differentiate function and structure of bacterial populations in soil, Fed. Biol. Res. Centre for Agriculture and Forestry,
Braunschweig, Germany
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1992 - 1993
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Teaching Biology, Microbiology and Waste water treatment,
Grone Bildungszentrum, Salzwedel, Germany
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1992
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Masters thesis (Diplom): Development and application of a method to characterize bacterial strains from oilfields by means of volatile products of their energy metabolism, University of Braunschweig, Germany |
1985 - 1991
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University education in General Biology, Main subjects: Microbiology, Biochemistry, Sanitary environmental engineering, University of Braunschweig, Germany |
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Research interests |
Molecular microbial ecology, sediment microbiology, anaerobic metabolism, subsurface of tidal flats, deep biosphere research, bacteriophages in deep sediments |
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Applied methods |
Microbial diversity assesement via PCR-DGGE of 16S rRNA (genes) and key genes indicative for metabolic processes, quantification of microorganisms and physiological groups by qPCR and CARD-FISH, molecular guided cultivation of anaerobes, activity measurements (exoenzymes, sulfate reduction rates), stable-isotope-probing |
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Projects |
DFG Collaborative Research Centre (Transregio-SFB 51) Roseobacter
PhD Research Training Group "The ecology of molecules" (EcoMol)
Collaborative Research Project "Assessment of ground- and porewater-derived nutrient fluxes into the German North Sea – Is there a ‘Barrier Island Mass Effect " (BIME)?
Deep sea drilling projects (ODP/IODP)
Meteor projects
DFG Research group BioGeoChemistry of tidal flats 2001-2011
Other projects
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Research
cruises |
Mai - Jun 2016
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Functional diversity of bacterial communities and the geometabolome in the central and north Pacific. (BacGeoPac) FS "SONNE" Expedition SO248 |
Mar - Apr 2012
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The Roseobacter clade and the DOM composition in the Drake Passage and the Antarctic Peninsula region of the Southern Ocean. FS "Polarstern" Expedition ANT28/4 |
Apr - Jun 2008
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Process studies in the eastern South Atlantic. RV "Meteor" Leg M76/1 |
Apr - Jun 2007
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Biogeochemistry and microbiology of the Black Sea and the regional paleo climate. RV "Meteor" Leg M72/5
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Jun - Aug 2004
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Juan De Fuca Hydrogeology. RV "JOIDES Resolution" IODP Exp. 301
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Nov - Dec 2001
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Characterization of bacteria in sapropels, in overlaying sediments and water layers by a multidisciplinary approach. RV "Meteor" Leg M51/3
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Oct 2001
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Microbial diversity at the chemocline of the Gotland deep. RV "Professor Albrecht Penck", Institute for Baltic Sea Research, Warnemünde, Germany |
Sep 1998
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Microbial diversity in the water column of the baltic sea. RV "Aranda", Finnish Institute of Marine Research, Helsinki, Finland |
Publications |
Profile on Google Scholar
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2018
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Castillo D, Vandieken V, Engelen B, Engelhardt T, Middelboe M (2018) Draft genome sequences of six Vibrio diazotrophicus strains isolated from deep subsurface sediments of the Baltic Sea. https://doi.org/10.1128/genomeA.00081-18
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Vandieken V, Marshall IPG, Niemann H, Engelen B, Cypionka H (2018) Labilibaculum manganireducens gen. nov., sp. nov. and Labilibaculum filiforme sp. nov., novel Bacteroidetes isolated from subsurface sediments of the Baltic Sea. Front Microbiol 8:2614, https://doi.org/10.3389/fmicb.2017.02614
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2017
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Beck M, Reckhardt A, Amelsberg J, Bartholomä A, Brumsack H-J, Cypionka H, Dittmar T, Engelen B, Greskowiak J, Hillebrand H, Holtappels M, Neuholz R, Köster J, Kuypers MMM, Massmann G, Meier D, Niggemann J, Paffrath R, Pahnke K, Rovo S, Striebel M, Vandieken V, Wehrmann A, Zielinski O (2017) The drivers of biogeochemistry in beach ecosystems: A cross-shore transect from the dunes to the low water line. Mar Chem, https://doi.org/10.1016/j.marchem.2017.01.001
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Harig T, Schlawis C, Ziesche L, Pohlner M, Engelen B, Schulz S (2017) Nitrogen-containing volatiles from marine Salinispora pacifica and Roseobacter-group bacteria. J Nat Prod ASAP, https://doi.org/10.1021/acs.jnatprod.7b00789
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Pohlner M, Degenhardt J, von Hoyningen-Huene AJE, Wemheuer B, Erlmann N, Schnetger B, Badewien TH, Engelen B (2017) The biogeographical distribution of benthic Roseobacter group members along a Pacific transect is structured by nutrient availability within the sediments and primary production in different oceanic provinces. Front Microbiol 8:2550, https://doi.org/10.3389/fmicb.2017.02550
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Pohlner M, Marshall I, Schreiber L, Cypionka H, Engelen B (2017) Draft genome sequence of Pseudoruegeria sp. SK021, a representative of the marine Roseobacter group, isolated from North Sea sediment. Genome Announc 5:e00541-17, https://doi.org/10.1128/genomeA.00541-17
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Vandieken V, Niemann H, Engelen B, Cypionka H (2017) Marinisporobacter balticus gen. nov., sp. nov., Desulfosporosinus nitroreducens sp. nov. and Desulfosporosinus fructosivorans sp. nov., new spore-forming bacteria isolated from subsurface sediments of the Baltic Sea. Int J Syst Evol Microbiol, 67:1887-1893, https://doi.org/10.1099/ijsem.0.001883
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Wöhlbrand L, Feenders C, Nachbaur J, Freund H, Engelen B, Wilkes H, Brumsack H, Rabus R (2017) Impact of extraction methods on the detectable protein complement of metaproteomic analyses of marine sediments. Proteomics 17:1700241 https://doi.org/10.1002/pmic.201700241
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2016
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Kanukollu S, Wemheuer B, Herber J, Billerbeck S, Lucas J, Daniel R, Simon M, Cypionka H, Engelen B (2016) Distinct compositions of free-living, particle-associated and benthic communities of the Roseobacter group in the North Sea. FEMS Microbiol Ecol, 92:(1), https://doi.org/10.1093/femsec/fiv145
| Kanukollu S, Voget S, Pohlner M, Vandieken V, Petersen J, Kyrpides NC, Woyke T, Shapiro N, Göker M, Klenk H-P, Cypionka H, Engelen B (2016). Genome sequence of Shimia str. SK013, a representative of the Roseobacter group isolated from marine sediment. SIGS,11:1-10, https://doi.org/10.1186/s40793-016-0143-0
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Satpathy P, Steinigeweg S, Cypionka H, Engelen B (2016) Different substrates and starter inocula govern microbial community structures in biogas reactors. Environ Technol 37:11, p. 1441-1450, https://doi.org/10.1080/09593330.2015.1118559
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2015
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Engelen B (2015) Prokaryotes and viruses in the marine subsurface:
From coastal sediments to the deep-subseafloor biosphere, Habilitation, Carl von Ossietzky Universität Oldenburg
| Fichtel K, Logemann J, Fichtel J, Rullkötter J, Cypionka H, Engelen B (2015) Temperature and pressure adaptation of a sulfate reducer from the deep subsurface. Front Microbiol, 6:1078, https://doi.org/10.3389/fmicb.2015.01078
| Kieft TL, Onstott TC, Ahonen L, Aloisi V, Colwell FS, Engelen B, Fendrihan S, Gaidos E, Harms U, Head I, Kallmeyer J, Kiel Reese B, Lin L-H, Long PE, Moser DP, Mills H, Sar P, Schulze-Makuch D, Stan-Lotter H, Wagner D, Wang P-L, Westall F, Wilkins MJ (2015) Workshop to develop deep-life continental scientific drilling projects. Sci. Dril., 3, 1-11, https://doi.org/10.5194/sd-19-43-2015
| Micic V, Köster J, Kruge MA, Engelen B, Hofmann T (2015) Bacterial wax esters in recent fluvial sediments. Org. Geochem. 88-89:44-66, https://doi.org/10.1016/j.orggeochem.2015.10.003
| Soora M, Tomasch J, Wang H, Michael V, Petersen J, Engelen B, Wagner-Doebler I and Cypionka H (2015) Oxidative stress and starvation in Dinoroseobacter shibae: The role of extrachromosomal elements. Front Microbiol 6:233. https://doi.org/10.3389/fmicb.2015.00233
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2014
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Engelen B, Imachi H (2014) Cultivation of subseafloor prokaryotic life. In: Stein R, Blackman DK, Inagaki F, Larsen H-C (Eds.), Earth and life processes discovered from subseafloor environments: A decade of science achieved by the Integrated Ocean Drilling Program (IODP), Elsevier: Developments in Marine Geology, vol. 7, chapt. 2.6, p. 197-215, ISSN 1572-5480, ISBN 9780444626172, https://doi.org/10.1016/B978-0-444-62617-2.00008-6
| Engelen B, Engelhardt T, Cypionka H (2014) Phagen in Sedimenten der marinen
tiefen Biosphäre. Biospektrum 04.14:380-382 https://doi.org/10.1007/s12268-014-0451-0
| Engelhardt T, Kallmeyer J, Cypionka H, Engelen B (2014) High virus-to-cell ratios indicate on-going production of viruses in deep subsurface sediments. ISME J 8:1503–1509, https://doi.org/10.1038/ismej.2013.245
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2013
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Engelhardt T, Sahlberg M, Cypionka H, Engelen B (2013) Biogeography of Rhizobium radiobacter and distribution of associated temperate phages in deep subseafloor sediments. ISME J 7:199-209 https://doi.org/110.1038/ismej.2012.92
| Kraft B, Engelen B, Goldhammer T, Lin Y-S, Cypionka H, Könneke M (2012) Desulfofrigus spec. prevails in sulfate-reducing dilution cultures from sediments of the Benguela upwelling area. FEMS Microbiol Ecol 84:86-97 https://doi.org/10.1111/1574-6941.12039
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2012
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Engelen B, Dogs M, Kanukollu S, Cypionka H (2012) The metabolic potential and the distribution of the Roseobacter clade in marine sediments. In: Lucassen M, with contributions of the participants (Eds.), The Expedition of the Research Vessel "Polarstern" to the Antarctic in 2012 (ANT-XXVIII/4), Reports on Polar and Marine Research, AWI Bremerhaven, p. 55-57, http://hdl.handle.net/10013/epic.40372, ISSN 1866-3192 https://epic.awi.de/31612/1/652-2012_Lucassen_ANT-28-4.pdf
| Engelen B, Kleindienst S, Lever M, Siegert M (2012) Microbiology: Molecular-guided cultivation assays, distribution and quantification of microbial communities, community zonation, activity, and genomics based on molecular methods. In: Zabel M, Boetius A, Emeis K-C, Ferdelman TG, Spieß V (Eds.), PROSA: Process Studies in the Eastern South Atlantic, Cruise No. 76, April 12 - August 24, 2008, METEOR-Berichte, Universität Hamburg, 1-33 - 1-40, ISSN 2195-8475
| Fichtel K, Mathes F, Könneke M, Cypionka H, Engelen B (2012) Isolation of sulfate-reducing bacteria from sediments above the deep-subseafloor aquifer. Front Microbio 3:65 https://doi.org/10.3389/fmicb.2012.00065
| Graue J, Kleindienst S, Lueders T, Cypionka H, Engelen B (2012) Identifying fermenting bacteria in anoxic tidal-flat sediments by a combination of microcalorimetry and ribosome-based stable-isotope probing. FEMS Microbiol Ecol 81:78–87 https://doi.org/10.1111/j.1574-6941.2011.01282.x
| Graue J, Engelen B, Cypionka H (2012) Degradation of cyanobacterial biomass in anoxic tidal-flat sediments: a microcosm study of metabolic processes and community changes. ISME J 6:660-669 https://doi.org/10.1038/ismej.2011.120
| Seidel M, Graue J, Engelen B, Köster J, Sass H, Rullkötter J (2012) Advection and diffusion determine vertical distribution of microbial communities in intertidal sediments as revealed by combined biogeochemical and molecular biological analysis. Org Geochem 52:114–129 https://doi.org/10.1016/j.orggeochem.2012.08.015
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2011
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Beck M, Riedel T, Graue J, Köster J, Kowalski N, Wu CS, Wegener G , Lipsewers Y, Freund H, Böttcher ME, Brumsack H-J, Cypionka H, Rullkötter J, Engelen B (2011) Imprint of past and present environmental conditions on microbiology and biogeochemistry of coastal quaternary sediments. Biogeosciences 8, 55-68 https://doi.org/10.5194/bg-8-55-2011
| Beckmann S, Lüders T, Krüger M, von Netzer F, Engelen B, Cypionka H (2011) Acetogens and acetoclastic methanosarcinales govern methane formation in abandoned coal mines. Appl. Environ. Microbiol. 77:3749–3756 https://doi.org/10.1128/AEM.02818-10
| Beckmann S, Krüger M, Engelen B, Gorbushina AA, Cypionka H (2011) Role of Bacteria, Archaea and Fungi involved in methane release in abandoned coal mines. Geomcrobiol J 28:347–358 https://doi.org/10.1080/01490451.2010.503258
| Cypionka H, Engelen B (2011) Tracing the Migration of Bacteria from the Water Column into Subsurface Sediments. In: Seifert R, Boetius A, Bohrmann G, Bialas J, Borowski C
(Eds.), Biogeochemistry, Microbiology, Marine Fluids, Gas Hydrates and Regional Palaeo-Climate of the Black Sea, Cruise No. 72, February 7 - June 4, 2007, METEOR-Berichte, Universität Hamburg, 12-1, 5-25 - 5-28, ISSN 0936-8957 https://doi.org/10.2312/cr_m72
| Engelhardt T, Sahlberg M, Cypionka H, Engelen B (2011) Induction of prophages from deep-subseafloor bacteria. Environm Microbiol Rep 3:459-465 https://doi.org/10.1111/j.1758-2229.2010.00232.x
| Logemann J, Graue J, Köster J, Engelen B, Rullkötter J, Cypionka H (2011) A laboratory experiment of intact polar lipid degradation in sandy sediments. Biogeosciences 8:2547-2560 https://doi.org/10.5194/bg-8-2547-2011
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2010
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Sass H, Köpke B, Rütters H, Feuerlein T, Dröge S, Cypionka H, Engelen B (2010) Tateyamaria pelophila sp. nov. a facultatively anaerobic Alphaproteobacterium isolated from tidal-flat sediments, and emended descriptions of the genus Tateyamaria and of Tateyamaria omphalii. IJSEM 60:1770-1777
| Steinsbu BO, Thorseth IH, Nakagawa S, Inagaki F, Lever MA, Engelen B, Ovreas L, Pedersen RB (2010) Archaeoglobus sulfaticallidus sp. nov., a novel thermophilic and facultatively lithoautotrophic sulfate-reducer isolated from black rust exposed to hot ridge flank crustal fluids. IJSEM 60:2745-2752
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2009
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Beck M, Köster J, Engelen B, Holstein JM, Gittel A, Könneke M, Riedel T, Wirtz K, Cypionka H, Rullkötter J, Brumsack H-J (2009) Deep pore water profiles reflect enhanced microbial activity towards tidal flat margins. Ocean Dynamics 59:371–384
| Engelen B and Cypionka H (2009) The subsurface of tidal flats as a model for the deep biosphere. Ocean Dynamics 59:385–391
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2008
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Engelen B, Ziegelmüller K, Wolf L, Köpke B, Gittel A, Treude T, Nakagawa S, Inagaki F, Lever MA, Steinsbu BO, Cypionka H (2008) Fluids from the oceanic crust support microbial activities within the deep biosphere. Geomicrobiol J 25:56-66
Krüger M, Beckmann S, Engelen B, Thielemann T, Cramer B, Schippers A, Cypionka H (2008) Microbial methane formation from hard coal and timber in an abandoned coal mine. Geomicrobiol J 25:315-321
Süß J, Herrmann K, Seidel M, Cypionka H, Engelen B, Sass H (2008) Two distinct Photobacterium populations thrive in ancient Mediterranean sapropels. Microb Ecol 55:371-383
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2007
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Batzke A, Engelen B, Sass H, Cypionka H (2007) Phylogenetic and physiological diversity of cultured deep-biosphere bacteria from Equatorial Pacific Ocean and Peru Margin sediments. Geomicrobiology J 24:261-273
Schmitz Y, Baurmann M, Engelen B, Feudel U (2007) Pattern Formation of Competing Microorganisms
in Sediments. Math Model Nat Phenom 2:74-104
Wilms R, Sass H, Köpke B, Cypionka H, Engelen B (2007) Methane and sulfate profiles within the subsurface of a tidal flat are reflected by the distribution of sulfate-reducing bacteria and methanogenic archaea. FEMS Microbiol Ecol 59:611621
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2006
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Lever MA, Alperin M, Engelen B, Inagaki F, Nakagawa S, Steinsbu BO, Teske A, IODP Expedition 301 Scientists (2006) Trends in basalt and sediment core contamination during IODP expedition 301. Geomicrobiol J 23:517-530
Nakagawa S, Inagaki F, Suzuki Y, Steinsbu BO, Lever MA, Takai K, Engelen B, Sako Y, Wheat CG, Horikoshi K, and IODP Exp. 301 Scientists (2006) Microbial Community in Black Rust Exposed to Hot Ridge Flank Crustal Fluids. Appl Environ Microbiol 72:67896799
Süß J, Schubert K, Sass H, Cypionka H, Overmann J, Engelen B (2006) Widespread distribution and high abundance of Rhizobium radiobacter within Mediterranean subsurface sediments. Environ Microbiol 8:1753-1763
Wilms R, Köpke B, Sass H, Chang TS, Cypionka H, Engelen B (2006) Deep biosphere-related bacteria within the subsurface of tidal flat sediments. Environ Microbiol 8:709-719
Wilms R, Sass H, Köpke B, Köster J, Cypionka H, Engelen B (2006) Specific bacterial, archaeal, and eukaryotic communities in tidal-flat sediment along a vertical profile of several meters. Appl Environ Microbiol 72:2756-2764
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2005
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Del Panno M, Morelli IS, Engelen B, Berthe-Corti L (2005) Effect of petrochemical sludge concentrations on microbial communities during a soil bioremediation process. FEMS Microbiol Ecol 53:305-316
Engelen B (2005) Im Untergrund des Watts: Bakterien in der "tiefen Biosphäre". Einblicke 41:25-27
Köpke B, Wilms R, Engelen B, Cypionka H, Sass H (2005) Microbial diversity in coastal subsurface sediments - a cultivation approach using various electron acceptors and substrate gradients. Appl Environ Microbiol 71:7819-7830
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2004
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D'Hondt S, Jørgensen BB, Miller DJ, Batzke A, Blake R, Cragg BA, Cypionka H, Dickens GR, Ferdelman T, Hinrichs KU, Holm NG, Mitterer R, Spivack A, Wang G, Bekins B, Engelen B, Ford K, Gettemy G, Rutherford SD, Sass H, Skilbeck CG, Aiello IW, Guèrin G, House C, Inagaki F, Meister P, Naehr T, Niitsuma S, Parkes RJ, Schippers A, Smith DC, Teske A, Wiegel J, Padilla CN, Acosta JLS (2004) Distributions of microbial activities in deep subseafloor sediments. Science 306:2216-2221
Süß J, Engelen B, Cypionka H, Sass H (2004) Quantitative analysis of bacterial communities from Mediterranean sapropels based on cultivation-dependent methods. FEMS Microbiol Ecol 51:109-121
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2003
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Sass H, Engelen B, Cypionka H (2003) Die tiefe Biosphäre - Mikrobiologie der Erdkruste. BioSpektrum 9:589-591
Overmann J, Schubert K, Hoffelner H, Engelen B (2003) Characterization of Bacteria in Sapropels, in Overlaying Sediments and Water Layers by a Multidisciplinary Approach. In: Hemleben C, Hoernle K, Jørgensen BB, and Roether W (Eds.), 2001, Ostatlantik - Mittelmeer - Schwarzes Meer, Cruise No. 51, 12 September - 28 December 2001. METEOR-Berichte, Universität Hamburg, 03-1, 225 pp. 3-32 - 3-40, ISSN 0936-8957
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Before 2000
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Engelen B (1998) Entwicklung und Anwendung von Methoden zur Differenzierung von Funktionen und Strukturen bakterieller Populationen des Bodens. Dissertation, TU Braunschweig
Glimm E, Heuer H, Engelen B, Smalla K, Backhaus H (1997) Statistical comparisons of community catabolic profiles. J Microbiol Meth 30:71-80.
Engelen B, Meinken K, von Wintzingerode F, Heuer H, Malkomes H-P, Backhaus H (1998) Monitoring Impact of a Pesticide Treatment on Bacterial Soil Communities by Metabolic and Genetic Fingerprinting in Addition to Conventional Testing Procedures. Appl Environ Microbiol 64:2814-2821
Felske A, Engelen B, Nübel U and Backhaus H (1996): Direct Ribosome Isolation from Soil to extract Bacterial rRNA for Community Analysis. Appl. Environ. Microbiol. 62: 4162-4167
Nübel U, Engelen B, Felske A, Snaidr L, Amann RI, Ludwig W and Backhaus H (1996): Sequence Heterogeneities of Genes encoding 16S rRNA in Paenibacillus polymyxa detected by Temperature Gel Electrophoresis. J. Bac. 178: 5636-5643
Backhaus H, Nickel A, Fritsch E, Huber J, Wendt-Potthoff K, Engelen B , Felske A , Nübel U and Jehle JA (1995): From Gene Transfer to Risk Assessment: Experiences with Genetic Variability in Baculoviruses and New Approaches to Analyse the Reaction of Microbial Communities to Stress Factors In: Schmidt et al. Hrsg. Springer, Berlin.
Heuer H, Hartung K, Engelen B and Smalla K (1995): Studies on microbial communities associated with potatoe plants by BIOLOG and TGGE patterns. Med. Fac. Landbouww. Univ. Gent, 60/4b, p.: 2639-2645
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